NQO1 monoclonal antibody (M01), clone 1E3-A6
产品名称: NQO1 monoclonal antibody (M01), clone 1E3-A6
英文名称: NQO1 monoclonal antibody (M01), clone 1E3-A6
产品编号: H00001728-M01
产品价格: null
产品产地: 台湾
品牌商标: Abnova
更新时间: null
使用范围:
亚诺法生技股份有限公司(Abnova)
- 联系人 :
- 地址 : 台湾台北市内湖区洲子街 108 号 9 楼
- 邮编 : 11493
- 所在区域 : 台湾
- 电话 : +886-920**1152 点击查看
- 传真 : 点击查看
- 邮箱 : sales@abnova.com.tw
- Specification
- Product Description:
- Mouse monoclonal antibody raised against a full length recombinant NQO1.
- Immunogen:
- NQO1 (AAH07659, 1 a.a. ~ 274 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
- Sequence:
- MVGRRALIVLAHSERTSFNYAMKEAAAAALKKKGWEVVESDLYAMNFNPIISRKDITGKLKDPANFQYPAESVLAYKEGHLSPDIVAEQKKLEAADLVIFQFPLQWFGVPAILKGWFERVFIGEFAYTYAAMYDKGPFRSKKAVLSITTGGSGSMYSLQGIHGDMNVILWPIQSGILHFCGFQVLEPQLTYSIGHTPADARIQILEGWKKRLENIWDETPLYFAPSSLFDLNFQAGFLMKKEVQDEEKNKKFGLSVGHHLGKSIPTDNQIKARK
- Host:
- Mouse
- Reactivity:
- Human
- Isotype:
- IgG1 Kappa
- Storage Buffer:
- In 1x PBS, pH 7.2
- Storage Instruction:
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
- Quality Control Testing:
- Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (55.88 KDa) .
- MSDS:
- Download
- Applications
- Western Blot (Cell lysate)
- NQO1 monoclonal antibody (M01), clone 1E3-A6 Western Blot analysis of NQO1 expression in HepG2 ( Cat # L019V1 ).
- Protocol Download
- Western Blot (Transfected lysate)
- Western Blot analysis of NQO1 expression in transfected 293T cell line by NQO1 monoclonal antibody (M01), clone 1E3-A6.
Lane 1: NQO1 transfected lysate(30.9 KDa).
Lane 2: Non-transfected lysate. - Protocol Download
- Western Blot (Recombinant protein)
- Protocol Download
- Immunofluorescence
- enlarge this image
- Immunofluorescence of monoclonal antibody to NQO1 on HepG2 cell. [antibody concentration 10 ug/ml]
- Protocol Download
- Immunoprecipitation
- Immunoprecipitation of NQO1 transfected lysate using anti-NQO1 monoclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with NQO1 MaxPab rabbit polyclonal antibody.
- Protocol Download
- Sandwich ELISA (Recombinant protein)
- Detection limit for recombinant GST tagged NQO1 is approximately 0.3ng/ml as a capture antibody.
- Protocol Download
- RNAi Knockdown (Antibody validated)
- Western blot analysis of NQO1 over-expressed 293 cell line, cotransfected with NQO1 Validated Chimera RNAi ( Cat # H00001728-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with NQO1 monoclonal antibody (M01), clone 1E3-A6 (Cat # H00001728-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
- Protocol Download
- Entrez GeneID:
- 1728
- GeneBank Accession#:
- BC007659
- Protein Accession#:
- AAH07659
- Gene Name:
- NQO1
- Gene Alias:
- DHQU,DIA4,DTD,NMOR1,NMORI,QR1
- Gene Description:
- NAD(P)H dehydrogenase, quinone 1
- Omim ID:
- 125860
- Gene Ontology:
- Hyperlink
- Gene Summary:
- This gene is a member of the NAD(P)H dehydrogenase (quinone) family and encodes a cytoplasmic 2-electron reductase. This FAD-binding protein forms homodimers and reduces quinones to hydroquinones. This protein's enzymatic activity prevents the one electron reduction of quinones that results in the production of radical species. Mutations in this gene have been associated with tardive dyskinesia (TD), an increased risk of hematotoxicity after exposure to benzene, and susceptibility to various forms of cancer. Altered expression of this protein has been seen in many tumors and is also associated with Alzheimer's disease (AD). Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq
- Other Designations:
- DT-diaphorase,NAD(P)H menadione oxidoreductase 1, dioxin-inducible,NAD(P)H:Quinone acceptor oxidoreductase type 1,NAD(P)H:menadione oxidoreductase 1,NAD(P)H:quinone oxireductase,azoreductase,diaphorase (NADH/NADPH) (cytochrome b-5 reductase),diaphorase-4,
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