DGKZ polyclonal antibody
产品名称： DGKZ polyclonal antibody
英文名称： DGKZ polyclonal antibody
产品编号： PAB3264
产品价格： null
产品产地： 台湾
品牌商标： Abnova
更新时间： null
使用范围：
亚诺法生技股份有限公司（Abnova)
- 联系人 :
- 地址 : 台湾台北市内湖区洲子街 108 号 9 楼
- 邮编 : 11493
- 所在区域 : 台湾
- 电话 : +886-920**1152 点击查看
- 传真 : 点击查看
- 邮箱 : sales@abnova.com.tw
- Specification
- Product Description:
- Rabbit polyclonal antibody raised against synthetic peptide of DGKZ.
- Immunogen:
- A synthetic peptide (conjugated with KLH) corresponding to N-terminus of human DGKZ.
- Host:
- Rabbit
- Reactivity:
- Human
- Form:
- Liquid
- Purification:
- Protein G purification
- Storage Buffer:
- In PBS (0.09% sodium azide)
- Storage Instruction:
- Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.
- Recommend Usage:
- ELISA (1:1000)
Western Blot (1:100-500)
Immunohistochemistry (1:50-100)
The optimal working dilution should be determined by the end user.
- Note:
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Publication Reference
- 1.
- Interaction of gamma 1-syntrophin with diacylglycerol kinase-zeta. Regulation of nuclear localization by PDZ interactions.
Hogan A, Shepherd L, Chabot J, Quenneville S, Prescott SM, Topham MK, Gee SH.J Biol Chem. 2001 Jul 13;276(28):26526-33. Epub 2001 May 14.
- 2.
- Protein kinase C regulates the nuclear localization of diacylglycerol kinase-zeta.
Topham MK, Bunting M, Zimmerman GA, McIntyre TM, Blackshear PJ, Prescott SM.Nature. 1998 Aug 13;394(6694):697-700.
- 3.
- Alternative splicing of the human diacylglycerol kinase zeta gene in muscle.
Ding L, Bunting M, Topham MK, McIntyre TM, Zimmerman GA, Prescott SM.Proc Natl Acad Sci U S A. 1997 May 27;94(11):5519-24.
- Applications
- Western Blot (Cell lysate)
- Western blot analysis of HepG2 cell lysate (35 ug/lane) with DGKZ polyclonal antibody (Cat # PAB3264) at 2 ug/mL.
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
- Formalin-fixed and paraffin-embedded human hepatocellular carcinoma tissue reacted with DGKZ polyclonal antibody (Cat # PAB3264) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. HC = hepatocarcinoma.
- Entrez GeneID:
- 8525
- Protein Accession#:
- Q13574
- Gene Name:
- DGKZ
- Gene Alias:
- DAGK5,DAGK6,DGK-ZETA,hDGKzeta
- Gene Description:
- diacylglycerol kinase, zeta 104kDa
- Omim ID:
- 601441
- Gene Ontology:
- Hyperlink
- Gene Summary:
- The protein encoded by this gene belongs to the eukaryotic diacylglycerol kinase family. It may attenuate protein kinase C activity by regulating diacylglycerol levels in intracellular signaling cascade and signal transduction. Alternative splicing occurs at this locus and four transcript variants encoding distinct isoforms have been identified. [provided by RefSeq
- Other Designations:
- diacylglycerol kinase zeta,diacylglycerol kinase, zeta (104kD)